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Bhat, A. R.
- Validation and Application of a High-Performance Liquid Chromatography Method for Estimation of Sitagliptin Phosphate from Bulk Drug and Pharmaceutical Formulation
Authors
1 Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, JNMC Campus, Nehrunagar, Belgaum-10, Karnataka, IN
2 Department of Pharmaceutical Analysis, Prin. K.M. Kundnani College of Pharmacy, Jote Joy Building, Cuffe Parade, Mumbai, IN
Source
Asian Journal of Research in Chemistry, Vol 5, No 1 (2012), Pagination: 123-126Abstract
A gradient LC method was developed for determination and quantitation of Sitagliptin in bulk drug and pharmaceutical formulations. The separation was accomplished on a symmetry reversed phase C18 column, 75 mm × 4.6mm I.D., 3.5μm column using mobile phase consist of acetonitrile and 0.03% formic acid at flow rate of 0.3ml/min. The eluents were monitored with a UV detector set at 268 nm as detection wavelength. The investigated validation elements showed the method has acceptable specificity, accuracy, linearity, precision, robustness. This method was found to be linear within range of 100 to 500 μg.ml-1 (r=0.9999). The method could be of use for rapid and routine quality control analysis of Sitagliptin. Thus, the developed method can be used for process development as well as quality assurance of Sitagliptin in bulk drug and pharmaceutical formulations.
Keywords
Sitagliptin Phosphate, RP-HPLC, Validation, Liquid Chromatography, Quality Assurance.- Synthesis of 1, 4 Benzothiazine Derivatives for Antimicrobial Activity
Authors
1 Department of Pharmaceutical Chemistry, K. L.E.U’s College of Pharmacy, Belgaum, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 11 (2011), Pagination: 1661-1663Abstract
Compound (3-oxo-3, 4-dihydro-2H-1, 4-benzothiazin-2-yl) acetic acid was synthesized by reacting o-Amino thiophenol and maleic anhydride. (3-oxo-3, 4-dihydro-2H-1, 4-benzothiazin-2-yl) acetate was prepared by Esterification of (3-oxo-3, 4-dihydro-2H-1, 4-benzothiazin-2-yl) acetic acid. Further eight Mannich bases were synthesized by using substituted Aromatic amines. The structures of synthesized compounds were confirmed on basis of IR, NMR and Mass Spectra. All synthesized compounds were screened for their antibacterial activity against strains viz. E. coli, E.fecalis, Klebshilla and S. aureus and antifungal activity against strains viz. C. albican and Aspergillus Niger. [4-({[4-( N-(5-methyl-4,5-dihydroisoxazol-3-yl)-aminosulfonyl) phenyl] amino} methyl)-3-oxo-3,4-dihydro- 2H-1,4-benzothiazin-2-yl]acetic acid, Ethyl[4-({[4-(N-(5-methyl-4,5-dihydro isoxazol-3-yl)-aminosulfonyl)phenyl] amino} methyl)-3-oxo-3,4-dihydro-2H-1,4-benzo thiazin-2-yl]acetate, [4-({[4-(N-pyridin-aminosulfonyl) phenyl] amino} methyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetic acid and Ethyl [4-({[4-(N-pyridin-aminosulfonyl) phenyl]amino}methyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetate have shown significant antibacterial and antifungal activity, while other derivatives shown moderate antibacterial and antifungal activity.Keywords
1, 4 Benzothiazine, 1, 4 Benzothiazine Ester, Antibacterial, Antifungal.- Validation and Application of a High-Performance Liquid Chromatography Method for Estimation of Sitagliptin Phosphate from Bulk Drug and Pharmaceutical Formulation
Authors
1 Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, JNMC Campus, Nehrunagar, Belgaum-10, Karnataka, IN
2 Department of Pharmaceutical Analysis, Prin. K.M. Kundnani College of Pharmacy, Jote Joy Building, Cuffe Parade, Mumbai, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 9 (2011), Pagination: 1466-1468Abstract
A gradient LC method was developed for determination and quantitation of Sitagliptin in bulk drug and pharmaceutical formulations. The separation was accomplished on a symmetry reversed phase C18 column, 75 mm ×4.6mm I.D., 3.5μm column using mobile phase consist of acetonitrile and 0.03% formic acid at flow rate of 0.3ml/min. The eluents were monitored with a UV detector set at 268 nm as detection wavelength. The investigated validation elements showed the method has acceptable specificity, accuracy, linearity, precision, robustness. This method was found to be linear within range of 100 to 500 μg.ml-1 (r=0.9999). The method could be of use for rapid and routine quality control analysis of Sitagliptin. Thus, the developed method can be used for process development as well as quality assurance of Sitagliptin in bulk drug and pharmaceutical formulations.Keywords
Sitagliptin Phosphate, RP-HPLC, Validation, Liquid Chromatography, Quality Assurance.- Simultaneous Estimation of Paracetamol, Caffeine, Pseudoephedrine Hydrochloride, Dextromethorphan Hydrobromide and Loratadine in Tablet Dosage form by RP-HPLC
Authors
1 Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, JNMC Campus, Nehrunagar, Belgaum-10, Karnataka, IN
2 Quality Assurance and Technical Services, Okasa Pharma Pvt. Ltd., Satara, Maharashtra, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 7 (2011), Pagination: 1141-1147Abstract
A simple, selective, rapid, precise and economical RP HPLC method has been developed for the determination of Paracetamol, Caffeine, Pseudoephedrine Hydrochloride, Dextromethorphan Hydrobromide and Loratadine in tablet formulation. The analysis was resolved by using a mobile phase (Sodium heptane sulphonate buffer solution and Acetonitrile in the ratio of 95:5) at a flow rate of 1ml/min on an gradient consisting of Shimadzu LC 2010 HPLC system on variable wavelength UV detector using, Inertsil ODS 3V (4.6 mm x 5 cm, 3 μm) column at a wavelength of 205 nm. The retention time were found to be Paracetamol (3.5min), Caffeine (5.5min), Pseudoephedrine HCl (8min), Dextromethorphan HBr (14min), Loratadine (15min). The percent recovery of Paracetamol, Caffeine, Pseudoephedrine Hydrochloride, Dextromethorphan Hydrobromide and Loratadine were found to be in between 98% to 102%. The developed method was simple, precise, accurate and reproducible and therefore suitable for routine analysis of drugs in tablet dosage form.
Keywords
Paracetamol, Caffeine, Pseudoephedrine HCl, Dextromethorphan HBr, Loratadine.- Development of RP-HPLC Method for Simultaneous Estimation of Amlodipine and Ramipril in Tablet Dosage Form
Authors
1 Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, Belgaum, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 8 (2011), Pagination: 1210-1213Abstract
Present work describes a precise, accurate and reproducible Reverse phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous estimation of Ramipril and Amlodipine in tablet dosage form on Phenomenex C18 column (245 mm × 4.5 mm, 5 μm, particle size) using acetonitrile: phosphate buffer, (60:40 v/v) as mobile phase at flow rate of 1.0 ml/min and the detection wavelength was 222 nm. The retention time for Ramipril and Amlodipine was found to be 3.41 and 6.10 min respectively. Proposed method was validated for precision, accuracy, linearity range, robustness and ruggedness.Keywords
Amlodipine, Ramipril, RP-HPLC.- Triazolone and their Derivatives for Anti-Tubercular Activities
Authors
1 KLE University’s College of Pharmacy, JNMC Campus, Belgaum-590 010, Karnataka, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 7 (2011), Pagination: 1050-1054Abstract
The Nitro containing 5-oxo-[1,2,4,]triazole ring were synthesized by the nitration of compound (I) with nitric acid. Then acetic acid ethyl esters containing 5-oxo-[1,2,4,]triazole ring (II) were synthesized by the condensation of compound (I) with ethyl chloroacetate in basic media. The reaction of compound (II) with hydrazine hydrate led to formation of acid hydrazide (III). The treatment of compound(III) with different aromatic aldhydes resulted in the formation of arylidene hydrazide's as target compounds. The structure of the synthesized Compounds were evaluated by IR, 1H NMR spectra and elemental method of analysis. All the synthesized compounds were screened for their antitubercular activity. Anti-tubercular activity was carried out by using middle brook 7H9 broth base (M198) medium against Mycobacterium tuberculosis of H37Rv Strain. Streptomycin was used as standard drug for comparison.Keywords
5-Oxo-[1,2,4,]Triazole, Acid Hydrazide, Arylidene Hydrazides, Anti-Tubercular Activities, Mycobacterium tuberculosis.- Synthesis and Evaluation of Benzimidazole Derivatives for Anti-Tubercular and Antimicrobial Activities
Authors
1 Department of Pharmaceutical Chemistry, K. L.E.U’s College of Pharmacy, Belgaum, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 7 (2011), Pagination: 1055-1058Abstract
The present research work was aimed to synthesize some benzimidazole acetic acid derivatives and also the derivatives associated with 1,2,4 triazolone and was investigated for their biological activities. A two different series of compounds were synthesized, 4-[(2-aryl methyl- 1H-benzimidazol-1-yl) acetyl] 5 nitro-2,4 -dihydro-3H-1,2,4 triazol- 3-one and 4-[(2-aryl methyl-1H-benzimidazol-1-yl)methyl] 5 nitro-2,4- dihydro-3H-1,2,4 triazol-3-one. The newly synthesized compounds have been characterized by their analytical and spectral (IR and 1H NMR, C13 NMR, mass spectra) properties. All the compounds have been screened for their anti tubercular and antimicrobial activities by standard methods. Results of activities reveal that compound shown promising anti tubercular activity at both the concentration compared to standard drug streptomycin, and shown good antimicrobial activities.Keywords
1,2,4 Triazolone, Benzimidazole, Anti Tubercular, Anti Bacterial , Antifungal Activity.- 4-Thiazolidinones as Calcium Antagonist:Synthesis and Pharmacological Screening
Authors
1 Fisher BioPharma Services (India) Pvt. Ltd, "PHARMEZ" SEZ, Plot No 22, Matoda, National Highway 8A, Ahmedabad-382213, Gujarat, IN
2 K.L.E.S’s College of Pharmacy, JNMC Campus, Belgaum-590010, Karnataka, IN